BayMax (Zeiss Axio Scan.Z1 Slide Scanner)

Automated slide scanner for fluorescence and brightfield stains, and the workhorse for anyone digitising a slide tray.

Configuration. Two cameras: an Orca Flash for fluorescence and an AxioCam IC colour camera for brightfield, both at 2048 x 2048. There are no lasers; excitation comes from filter cubes for DAPI (FS 49), eGFP (FS 38 HE), Cy3 (FS 43 HE), mPlum (FS 64 HE) and Cy5 (FS 50). Z-stacks are supported. Samples must be fixed and sealed slides, either 1x3 inch or 2x3 inch.

Objectives. 5x, 10x and 20x, all air.

Software. Zen Blue 2012, with Zen Lite available as a free viewer.