C-3PO (Bruker TruLive3D Imager Lightsheet)

The Bruker TruLive3D Imager is a lightsheet system built specifically for live 3D imaging, with the environmental control to keep a sample healthy across a long run.

Configuration. Hamamatsu Orca Flash 4.0 V3 camera. Imaging excitation at 445, 488, 515, 561, 642 and 685 nm, with photomanipulation at 405 nm and pulsed 532 nm. Environmental control covers heating, cooling, CO2 and N2. Z-stacks and time lapse are supported. Cleared samples are not.

Optics. A 25x/1.1 Nikon water objective with 2 mm working distance, plus a 0.5x, 1.0x, 1.5x and 2.0x magnification changer giving 12.5x to 50x. Dichroics: LP 499, LP 560, LP 653. Short-camera filters: BP 457-501, BP 497-554, BP 500-530, BP 526-564, BP 580-627, BP 610-629, with four empty slots. Long-camera filters: BP 497-554, BP 500-530 and others.