Morpheus (Zeiss Lightsheet for CLARITY)

Installed in February 2015, Morpheus is the MIC's cleared-tissue lightsheet: a Zeiss lightsheet carrying optics for both water and cleared samples at refractive index around 1.45. For live, water-based work, Serenity is the better choice.

Configuration. Selective plane illumination with excitation at 405, 440, 488, 514, 561 and 642 nm, and IR-LED brightfield. Z-stacks and time lapse are supported. Cleared samples are handled with the 20x or 5x CLARITY objectives. Live imaging is possible, though Serenity is recommended for water-based live work. A paired offline workstation running Arivis handles rendering and analysis; it is listed separately as Morpheus Processing.

Objectives. 20x CLARITY, 20x water, and 5x CLARITY.

Software. Zen Black LS. Laser use authorization 4154.